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Zhongguo Yao Li Xue Bao. 1997 Nov;18(6):515-8.

Effects of berberine of L- and T-type calcium channels in guinea pig ventricular myocytes.

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  • 1Cardiovascular Research Laboratory, Shanghai Xu-hui Central Hospital, China.

Abstract

AIM:

To study the effects of berberine (Ber) on L-(ICa,L) and T-type (ICa,T) channels in isolated guinea pig ventricular myocytes.

METHODS:

Using whole cell patch-clamp recording technique.

RESULTS:

Ber 10, 30 mumol.L-1 inhibited the ICa, L from 1400 +/- 247 pA to 978 +/- 204 pA (n = 5 cells of 5 guinea pigs, P < 0.05), and to 664 +/- 179 pA (n = 5, P < 0.01), respectively. The inhibitory effect was concentration-dependent and non-frequency-dependent. The peak value of ICa,L in the current-voltage relationship was decreased. Ber affected the inactivation kinetics of ICa,L. The half activation potential (V0.5) was shifted from -27.8 mV to -34.2 mV and the slope factor (kappa) was changed from 9.22 into 13.03. Ber did not affect the activation kinetics. Ber 10 and 30 mumol.L-1 also inhibited ICa,T (from 154 +/- 80 pA to 101 +/- 78 pA, and to 48 +/- 45 pA, n = 8 cells of 5 guinea pigs, P < 0.05).

CONCLUSION:

Ber possessed blocking effects on both L- and T-type calcium channels.

PMID:
10322908
[PubMed - indexed for MEDLINE]
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