EMBO J. 1999 May 4;18(9):2621-30.

Molecular cloning of the maize gene crp1 reveals similarity between regulators of mitochondrial and chloroplast gene expression.

Source

Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA.

Abstract

The maize nuclear gene crp1 is required for the translation of the chloroplast petA and petD mRNAs and for the processing of the petD mRNA from a polycistronic precursor. In order to understand the biochemical role of the crp1 gene product and the interconnections between chloroplast translation and RNA metabolism, the crp1 gene and cDNA were cloned. The predicted crp1 gene product (CRP1) is related to nuclear genes in fungi that play an analogous role in mitochondrial gene expression, suggesting an underlying mechanistic similarity. Analysis of double mutants that lack both chloroplast ribosomes and crp1 function indicated that CRP1 activates a site-specific endoribonuclease independently of any role it plays in translation. Antibodies prepared to recombinant CRP1 were used to demonstrate that CRP1 is localized to the chloroplast stroma and that it is a component of a multisubunit complex. The CRP1 complex is not associated detectably with either chloroplast membranes or chloroplast ribosomes. Models for CRP1 function and its relationship to other activators of organellar translation are discussed.

PMID:: 10228173; [PubMed - indexed for MEDLINE]
PMCID: PMC1171341

Free PMC Article

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

GENBANK/AF073522

Grant Support

5 T32 GM07759/GM/NIGMS NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

Saccharomyces Genome Database

Supplemental Content

Related citations

A nuclear mutation in maize blocks the processing and translation of several chloroplast mRNAs and provides evidence for the differential translation of alternative mRNA forms. [EMBO J. 1994]
A nuclear mutation in maize blocks the processing and translation of several chloroplast mRNAs and provides evidence for the differential translation of alternative mRNA forms.
Barkan A, Walker M, Nolasco M, Johnson D. EMBO J. 1994 Jul 1; 13(13):3170-81.
HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs. [Plant Cell. 2003]
HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs.
Meierhoff K, Felder S, Nakamura T, Bechtold N, Schuster G. Plant Cell. 2003 Jun; 15(6):1480-95.
RNA immunoprecipitation and microarray analysis show a chloroplast Pentatricopeptide repeat protein to be associated with the 5' region of mRNAs whose translation it activates. [Plant Cell. 2005]
RNA immunoprecipitation and microarray analysis show a chloroplast Pentatricopeptide repeat protein to be associated with the 5' region of mRNAs whose translation it activates.
Schmitz-Linneweber C, Williams-Carrier R, Barkan A. Plant Cell. 2005 Oct; 17(10):2791-804. Epub 2005 Sep 2.
A SecY homologue is required for the elaboration of the chloroplast thylakoid membrane and for normal chloroplast gene expression. [J Cell Biol. 1998]
A SecY homologue is required for the elaboration of the chloroplast thylakoid membrane and for normal chloroplast gene expression.
Roy LM, Barkan A. J Cell Biol. 1998 Apr 20; 141(2):385-95.
Nuclear genes required for post-translational steps in the biogenesis of the chloroplast cytochrome b6f complex in maize. [Mol Gen Genet. 1995]
Nuclear genes required for post-translational steps in the biogenesis of the chloroplast cytochrome b6f complex in maize.
Voelker R, Barkan A. Mol Gen Genet. 1995 Dec 15; 249(5):507-14.

See reviews... See all...

Cited by 66 PubMed Central articles

Recruitment of a ribosomal release factor for light- and stress-dependent regulation of petB transcript stability in Arabidopsis chloroplasts. [Plant Cell. 2011]
Recruitment of a ribosomal release factor for light- and stress-dependent regulation of petB transcript stability in Arabidopsis chloroplasts.
Stoppel R, Lezhneva L, Schwenkert S, Torabi S, Felder S, Meierhoff K, Westhoff P, Meurer J. Plant Cell. 2011 Jul; 23(7):2680-95. Epub 2011 Jul 19.
The nucleus-encoded trans-acting factor MCA1 plays a critical role in the regulation of cytochrome f synthesis in Chlamydomonas chloroplasts. [Plant Cell. 2011]
The nucleus-encoded trans-acting factor MCA1 plays a critical role in the regulation of cytochrome f synthesis in Chlamydomonas chloroplasts.
Boulouis A, Raynaud C, Bujaldon S, Aznar A, Wollman FA, Choquet Y. Plant Cell. 2011 Jan; 23(1):333-49. Epub 2011 Jan 7.
Selection of Shine-Dalgarno sequences in plastids. [Nucleic Acids Res. 2011]
Selection of Shine-Dalgarno sequences in plastids.
Drechsel O, Bock R. Nucleic Acids Res. 2011 Mar; 39(4):1427-38. Epub 2010 Oct 21.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Molecular cloning of the maize gene crp1 reveals similarity between regulators o...
Molecular cloning of the maize gene crp1 reveals similarity between regulators of mitochondrial and chloroplast gene expression.
EMBO J. 1999 May 4 ;18(9):2621-30.

PubMed
Use of botulinum toxin type A injection for neuropathic pain after trigeminal ne...
Use of botulinum toxin type A injection for neuropathic pain after trigeminal nerve injury.
Pain Med. 2010 Apr ;11(4):630-2. Epub 2010 Mar 4 .

PubMed
From endothelial dysfunction to atherosclerosis.
From endothelial dysfunction to atherosclerosis.
Autoimmun Rev. 2010 Oct ;9(12):830-4. Epub 2010 Jul 30 .

PubMed
MafA and MafB regulate genes critical to beta-cells in a unique temporal manner.
MafA and MafB regulate genes critical to beta-cells in a unique temporal manner.
Diabetes. 2010 Oct ;59(10):2530-9. Epub 2010 Jul 13 .

PubMed
Mitigating risky sexual behaviors among Russian narcology hospital patients: the...
Mitigating risky sexual behaviors among Russian narcology hospital patients: the PREVENT (Partnership to Reduce the Epidemic Via Engagement in Narcology Treatment) randomized controlled trial.
Addiction. 2008 Sep ;103(9):1474-83. Epub 2008 Jul 10 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: