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    J Chromatogr B Biomed Sci Appl. 1999 Mar 19;724(2):389-92.

    Determination of trans-resveratrol in human plasma by high-performance liquid chromatography.

    Zhu Z, Klironomos G, Vachereau A, Neirinck L, Goodman DW.

    Pharmascience, Montreal, Quebec, Canada. zzhu@pharmascience.com

    The first method using high-performance liquid chromatography (HPLC) has been developed for the determination of trans-resveratrol in human plasma. The method involves a liquid--liquid extraction followed by reversed-phase HPLC with UV detection. The detection limit of trans-resveratrol in human plasma was 5.0 ng/ml. Standard curves are linear over the concentration range of 5.0-5000.0 ng/ml. Intra-assay variability ranged from 1.9 to 3.7% and inter-assay variability ranged from 2.5 to 4.0% at the concentration range of 15.0-4000.0 ng/ml.

    PMID: 10219682 [PubMed - indexed for MEDLINE]

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