Nucleic Acids Res. 1999 May 15;27(10):2165-74.

Isolation of Ku70-binding proteins (KUBs).

Yang CR, Yeh S, Leskov K, Odegaard E, Hsu HL, Chang C, Kinsella TJ, Chen DJ, Boothman DA.

Source

Department of Radiation Oncology and Department of Pharmacology and the Ireland Cancer Center,Laboratory of Molecular Stress Responses, Case Western Reserve University, BRB-326 East,10900 Euclid Avenue, Cleveland, OH 44106-4942, USA.

Abstract

DNA-dependent protein kinase (DNA-PK) plays a critical role in resealing DNA double-stand breaks by non-homologous end joining. Aside from DNA-PK, XRCC4 and DNA ligase IV, other proteins which play a role(s) in this repair pathway remain unknown; DNA-PK contains a catalytic subunit (DNA-PKcs) and a DNA binding subunit (Ku70 and Ku80). We isolated Ku70-binding proteins (KUB1-KUB4) using yeast two-hybrid analyses. Sequence analyses revealed KUB1 to be apolipoprotein J (apoJ), also known as X-ray-inducible transcript 8 (XIP8), testosterone-repressed prostate message-2 (TRPM-2) and clusterin. KUB2 is Ku80. KUB3 and KUB4 are unknown, >10 kb trans-cripts. Interactions of apoJ/XIP8 or KUB3 with Ku70 were confirmed by co-immunoprecipitation analyses in MCF-7:WS8 breast cancer or IMR-90 normal lung fibroblast cells, respectively. The interaction of apoJ/XIP8 with Ku70 was confirmed by far-western analyses. Stable over-expression of full-length apoJ/XIP8 in MCF-7:WS8 caused decreased Ku70/Ku80 DNA end binding that was restored by apoJ/XIP8 monoclonal antibodies. The role of apoJ/XIP8 in ionizing radiation resistance/sensitivity is under investigation.

PMID:: 10219089; [PubMed - indexed for MEDLINE]
PMCID:: PMC148436

Free PMC Article

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Nuclear clusterin/XIP8, an x-ray-induced Ku70-binding protein that signals cell death. [Proc Natl Acad Sci U S A. 2000]
Nuclear clusterin/XIP8, an x-ray-induced Ku70-binding protein that signals cell death.
Yang CR, Leskov K, Hosley-Eberlein K, Criswell T, Pink JJ, Kinsella TJ, Boothman DA. Proc Natl Acad Sci U S A. 2000 May 23; 97(11):5907-12.
A central region of Ku80 mediates interaction with Ku70 in vivo. [Nucleic Acids Res. 1998]
A central region of Ku80 mediates interaction with Ku70 in vivo.
Cary RB, Chen F, Shen Z, Chen DJ. Nucleic Acids Res. 1998 Feb 15; 26(4):974-9.
Specific interaction of IP6 with human Ku70/80, the DNA-binding subunit of DNA-PK. [EMBO J. 2002]
Specific interaction of IP6 with human Ku70/80, the DNA-binding subunit of DNA-PK.
Hanakahi LA, West SC. EMBO J. 2002 Apr 15; 21(8):2038-44.
Review When X-ray-inducible proteins meet DNA double strand break repair. [Semin Radiat Oncol. 2001]
Review When X-ray-inducible proteins meet DNA double strand break repair.
Leskov KS, Criswell T, Antonio S, Li J, Yang CR, Kinsella TJ, Boothman DA. Semin Radiat Oncol. 2001 Oct; 11(4):352-72.
Review Colworth Medal lecture. Detection, repair and signalling of DNA double-strand breaks. [Biochem Soc Trans. 1999]
Review Colworth Medal lecture. Detection, repair and signalling of DNA double-strand breaks.
Jackson SP. Biochem Soc Trans. 1999 Feb; 27(2):1-13.

See reviews... See all...

Cited by 13 PubMed Central articles

Exon-skipping strategy by ratio modulation between cytoprotective versus pro-apoptotic clusterin forms increased sensitivity of LNCaP to cell death. [PLoS One. 2013]
Exon-skipping strategy by ratio modulation between cytoprotective versus pro-apoptotic clusterin forms increased sensitivity of LNCaP to cell death.
Essabbani A, Garcia L, Zonetti MJ, Fisco T, Pucci S, Chiocchia G. PLoS One. 2013; 8(2):e54920. Epub 2013 Feb 13.
Tumor biomarker glycoproteins in the seminal plasma of healthy human males are endogenous ligands for DC-SIGN. [Mol Cell Proteomics. 2012]
Tumor biomarker glycoproteins in the seminal plasma of healthy human males are endogenous ligands for DC-SIGN.
Clark GF, Grassi P, Pang PC, Panico M, Lafrenz D, Drobnis EZ, Baldwin MR, Morris HR, Haslam SM, Schedin-Weiss S, et al. Mol Cell Proteomics. 2012 Jan; 11(1):M111.008730. Epub 2011 Oct 10.
CRM1 protein-mediated regulation of nuclear clusterin (nCLU), an ionizing radiation-stimulated, Bax-dependent pro-death factor. [J Biol Chem. 2011]
CRM1 protein-mediated regulation of nuclear clusterin (nCLU), an ionizing radiation-stimulated, Bax-dependent pro-death factor.
Leskov KS, Araki S, Lavik JP, Gomez JA, Gama V, Gonos ES, Trougakos IP, Matsuyama S, Boothman DA. J Biol Chem. 2011 Nov 18; 286(46):40083-90. Epub 2011 Sep 27.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
Gene (nucleotide/PMC)
Records in Gene identified from shared sequence and PMC links.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Isolation of Ku70-binding proteins (KUBs).
Isolation of Ku70-binding proteins (KUBs).
Nucleic Acids Res. 1999 May 15 ;27(10):2165-74.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Isolation of Ku70-binding proteins (KUBs).

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Research Materials

Supplemental Content

Save items

Related citations in PubMed

Cited by 13 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information