Format

Send to:

Choose Destination
See comment in PubMed Commons below
Curr Med Chem. 1999 Jun;6(6):481-99.

Transmembrane pH gradients in vivo: measurements using fluorinated vitamin B6 derivatives.

Author information

  • 1Department of Radiology, U.T. Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9058, USA.

Abstract

It is well recognized that pH plays a significant regulatory role in most cellular processes. Increasingly, there is interest in transmembrane pH gradients, particularly with respect to tumor growth and response to therapy. NMR offers a non-invasive approach to monitoring cellular pH and detecting changes in response to interventions. This review will consider the strengths of various approaches to measuring pH with particular focus on the reporter molecules designed to interrogate the cellular milieu. In particular, fluorinated vitamin B6 derivatives (6-fluoropyridoxol and 6-fluoropyridoxamine) will be described, which for the first time provide a practical non-destructive method to measure simultaneously intra- and extracellular pH, i.e., the transmembrane pH gradient in animals in vivo based on a single reporter molecule.

PMID:
10213795
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk