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Curr Opin Biotechnol. 1999 Apr;10(2):156-9.

Large-scale transient expression in mammalian cells for recombinant protein production.

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  • 1Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.


Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.

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