Trends Cell Biol. 1999 Apr;9(4):154-9.

High-resolution electron cryomicroscopy of macromolecular assemblies.

Chiu W, McGough A, Sherman MB, Schmid MF.

Source

Verna and Marrs McLean Dept of Biochemistry and National Center for Macromolecular Imaging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA. wah@bcm.tmc.edu

Abstract

Electron cryomicroscopy is a high-resolution imaging technique that is particularly appropriate for the structural determination of large macromolecular assemblies, which are difficult to study by X-ray crystallography or NMR spectroscopy. For some biological molecules that form two-dimensional crystals, the application of electron cryomicroscopy and image reconstruction can help elucidate structures at atomic resolution. In instances where crystals cannot be formed, atomic-resolution information can be obtained by combining high-resolution structures of individual components determined by X-ray crystallography or NMR with image-derived reconstructions at moderate resolution. This can provide unique and crucial information on the mechanisms of these complexes. Finally, image reconstructions can be used to augment X-ray studies by providing initial models that facilitate phasing of crystals of large macromolecular machines such as ribosomes and viruses.

PMID:: 10203794; [PubMed - indexed for MEDLINE]

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Macromolecular Substances

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P41RR02250/RR/NCRR NIH HHS/United States

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