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1: J Clin Microbiol. 1999 May;37(5):1503-9.Click here to read Click here to read Links

Direct quantification of the enteric bacterium Oxalobacter formigenes in human fecal samples by quantitative competitive-template PCR.

Sidhu H, Holmes RP, Allison MJ, Peck AB.

Ixion Biotechnology, Inc., Alachua, Florida. sidhu@biotech.ufl.org

Homeostasis of oxalic acid appears to be regulated, in part, by the gut-associated bacterium Oxalobacter formigenes. The loss of this bacterium from the gut flora is associated with an increased susceptibility to hyperoxaluria, a condition which can lead to the formation of calcium oxalate crystalluria and kidney stones. In order to identify and quantify the presence of O. formigenes in clinical specimens, a quantitative-PCR-based assay system utilizing a competitive DNA template as an internal standard was developed. This quantitative competitive-template PCR test allows for the rapid, highly specific, and reproducible quantification of O. formigenes in fecal samples and provides a prototype for development of DNA-based quantitative assays for enteric bacteria.

PMID: 10203513 [PubMed - indexed for MEDLINE]

PMCID: PMC84815