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Fertil Steril. 1999 Apr;71(4):719-21.

The first polar body does not predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes.

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  • 1Department of Obstetrics and Gynecology, Women and Infants Hospital, Brown University School of Medicine, Providence, Rhode Island 02905, USA.

Abstract

OBJECTIVE:

To evaluate how well polar body location predicts spindle localization and to examine spindle morphology.

DESIGN:

Randomized, controlled animal study.

SETTING:

University-affiliated research laboratory.

ANIMAL(S):

Mature, female golden hamsters.

INTERVENTION(S):

After superovulation with pregnant mare serum gonadotropin and hCG, metaphase II oocytes were obtained and imaged under digital polarization microscopy.

MAIN OUTCOME MEASURE(S):

Identify the meiotic spindle in living, unfixed hamster oocytes and determine spindle location relative to the polar body.

RESULT(S):

Spindles were imaged in 30 oocytes and only in 5 of them could the polar body predict the spindle localization. In the remaining oocytes, the spindles presented a random distribution within the cytoplasm.

CONCLUSION(S):

These data show that the polar body location is not an accurate predictor for meiotic spindle location in mammalian oocytes.

PMID:
10202885
[PubMed - indexed for MEDLINE]

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