EMBO J. 1999 Apr 1;18(7):1923-38.

Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31.

Aagaard L, Laible G, Selenko P, Schmid M, Dorn R, Schotta G, Kuhfittig S, Wolf A, Lebersorger A, Singh PB, Reuter G, Jenuwein T.

Source

Research Institute of Molecular Pathology (IMP), The Vienna Biocenter, Dr. Bohrgasse 7, A-1030 Vienna, Austria.

Abstract

The chromo and SET domains are conserved sequence motifs present in chromosomal proteins that function in epigenetic control of gene expression, presumably by modulating higher order chromatin. Based on sequence information from the SET domain, we have isolated human (SUV39H1) and mouse (Suv39h1) homologues of the dominant Drosophila modifier of position-effect-variegation (PEV) Su(var)3-9. Mammalian homologues contain, in addition to the SET domain, the characteristic chromo domain, a combination that is also preserved in the Schizosaccharyomyces pombe silencing factor clr4. Chromatin-dependent gene regulation is demonstrated by the potential of human SUV39H1 to increase repression of the pericentromeric white marker gene in transgenic flies. Immunodetection of endogenous Suv39h1/SUV39H1 proteins in a variety of mammalian cell lines reveals enriched distribution at heterochromatic foci during interphase and centromere-specific localization during metaphase. In addition, Suv39h1/SUV39H1 proteins associate with M31, currently the only other characterized mammalian SU(VAR) homologue. These data indicate the existence of a mammalian SU(VAR) complex and define Suv39h1/SUV39H1 as novel components of mammalian higher order chromatin.

PMID:: 10202156; [PubMed - indexed for MEDLINE]
PMCID:: PMC1171278

Free PMC Article

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Secondary Source ID

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Research Materials

Anti-KMT1A / SUV39H1 antibody [44.1] - ChIP Grade - Abcam plc

Miscellaneous

Mouse Genome Informatics (MGI)

Supplemental Content

Save items

Related citations in PubMed

Structure-function analysis of SUV39H1 reveals a dominant role in heterochromatin organization, chromosome segregation, and mitotic progression. [Mol Cell Biol. 2000]
Structure-function analysis of SUV39H1 reveals a dominant role in heterochromatin organization, chromosome segregation, and mitotic progression.
Melcher M, Schmid M, Aagaard L, Selenko P, Laible G, Jenuwein T. Mol Cell Biol. 2000 May; 20(10):3728-41.
Mitotic phosphorylation of SUV39H1, a novel component of active centromeres, coincides with transient accumulation at mammalian centromeres. [J Cell Sci. 2000]
Mitotic phosphorylation of SUV39H1, a novel component of active centromeres, coincides with transient accumulation at mammalian centromeres.
Aagaard L, Schmid M, Warburton P, Jenuwein T. J Cell Sci. 2000 Mar; 113 ( Pt 5):817-29.
Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing. [EMBO J. 2002]
Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing.
Schotta G, Ebert A, Krauss V, Fischer A, Hoffmann J, Rea S, Jenuwein T, Dorn R, Reuter G. EMBO J. 2002 Mar 1; 21(5):1121-31.
Review Histone modification and the control of heterochromatic gene silencing in Drosophila. [Chromosome Res. 2006]
Review Histone modification and the control of heterochromatic gene silencing in Drosophila.
Ebert A, Lein S, Schotta G, Reuter G. Chromosome Res. 2006; 14(4):377-92.
Review SU(VAR)3-9 is a conserved key function in heterochromatic gene silencing. [Genetica. 2003]
Review SU(VAR)3-9 is a conserved key function in heterochromatic gene silencing.
Schotta G, Ebert A, Reuter G. Genetica. 2003 Mar; 117(2-3):149-58.

See reviews... See all...

Cited by 93 PubMed Central articles

Mealybug chromosome cycle as a paradigm of epigenetics. [Genet Res Int. 2012]
Mealybug chromosome cycle as a paradigm of epigenetics.
Prantera G, Bongiorni S. Genet Res Int. 2012; 2012:867390. Epub 2012 Apr 8.
Heterochromatin influences the secondary metabolite profile in the plant pathogen Fusarium graminearum. [Fungal Genet Biol. 2012]
Heterochromatin influences the secondary metabolite profile in the plant pathogen Fusarium graminearum.
Reyes-Dominguez Y, Boedi S, Sulyok M, Wiesenberger G, Stoppacher N, Krska R, Strauss J. Fungal Genet Biol. 2012 Jan; 49(1):39-47. Epub 2011 Nov 11.
ncRNA- and Pc2 methylation-dependent gene relocation between nuclear structures mediates gene activation programs. [Cell. 2011]
ncRNA- and Pc2 methylation-dependent gene relocation between nuclear structures mediates gene activation programs.
Yang L, Lin C, Liu W, Zhang J, Ohgi KA, Grinstein JD, Dorrestein PC, Rosenfeld MG. Cell. 2011 Nov 11; 147(4):773-88.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Domains
Conserved Domain Database (CDD) records that cite the current articles. Citations are from the CDD source database records (PFAM, SMART).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode...
Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31.
EMBO J. 1999 Apr 1 ;18(7):1923-38.

PubMed
Updated bronchodilator analysis.
Updated bronchodilator analysis.
Arch Pediatr Adolesc Med. 1999 Apr ;153(4):430.

PubMed
Reducing obesity via a school-based interdisciplinary intervention among youth: ...
Reducing obesity via a school-based interdisciplinary intervention among youth: Planet Health.
Arch Pediatr Adolesc Med. 1999 Apr ;153(4):409-18.

PubMed
Celiac disease and markers of celiac disease latency in patients with primary Sj...
Celiac disease and markers of celiac disease latency in patients with primary Sjögren's syndrome.
Am J Gastroenterol. 1999 Apr ;94(4):1042-6.

PubMed
Pain: the overlooked symptom in gastroparesis.
Pain: the overlooked symptom in gastroparesis.
Am J Gastroenterol. 1999 Apr ;94(4):1029-33.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: