Appl Environ Microbiol. 1999 Apr;65(4):1786-8.

Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots.

Johnsen K, Enger O, Jacobsen CS, Thirup L, Torsvik V.

Source

Geological Survey of Denmark and Greenland, Copenhagen, Denmark. kj@geus.dk

Abstract

We used a quantitative PCR method targeting 16S ribosomal DNA using competitive PCR for specific detection of indigenous Pseudomonas DNA in soil hot spots. The amount of Pseudomonas DNA corresponded to the number of culturable Pseudomonas bacteria on Gould's S1 agar. This represents the first use of PCR for quantification of indigenous bacteria in more than one sample of soil.

PMID:: 10103283; [PubMed - indexed for MEDLINE]
PMCID:: PMC91253

Free PMC Article

Images from this publication.See all images (3) Free text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Diversity of Pseudomonas strains isolated with King's B and Gould's S1 agar determined by repetitive extragenic palindromic-polymerase chain reaction, 16S rDNA sequencing and Fourier transform infrared spectroscopy characterisation. [FEMS Microbiol Lett. 1999]
Diversity of Pseudomonas strains isolated with King's B and Gould's S1 agar determined by repetitive extragenic palindromic-polymerase chain reaction, 16S rDNA sequencing and Fourier transform infrared spectroscopy characterisation.
Johnsen K, Nielsen P. FEMS Microbiol Lett. 1999 Apr 1; 173(1):155-62.
Quantification of the Pseudomonas population in New Zealand soils by fluorogenic PCR assay and culturing techniques. [J Microbiol Methods. 2005]
Quantification of the Pseudomonas population in New Zealand soils by fluorogenic PCR assay and culturing techniques.
Lloyd-Jones G, Laurie AD, Tizzard AC. J Microbiol Methods. 2005 Feb; 60(2):217-24.
An altered Pseudomonas diversity is recovered from soil by using nutrient-poor Pseudomonas-selective soil extract media. [Appl Environ Microbiol. 2001]
An altered Pseudomonas diversity is recovered from soil by using nutrient-poor Pseudomonas-selective soil extract media.
Aagot N, Nybroe O, Nielsen P, Johnsen K. Appl Environ Microbiol. 2001 Nov; 67(11):5233-9.
Specific PCR amplification for the genus Pseudomonas targeting the 3' half of 16S rDNA and the whole 16S-23S rDNA spacer. [Syst Appl Microbiol. 2002]
Specific PCR amplification for the genus Pseudomonas targeting the 3' half of 16S rDNA and the whole 16S-23S rDNA spacer.
Locatelli L, Tarnawski S, Hamelin J, Rossi P, Aragno M, Fromin N. Syst Appl Microbiol. 2002 Aug; 25(2):220-7.
Review Physiology of the bdellovibrios. [Adv Microb Physiol. 1972]
Review Physiology of the bdellovibrios.
Starr MP, Huang JC. Adv Microb Physiol. 1972; 8:215-61.

See reviews... See all...

Cited by 14 PubMed Central articles

Quantification of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens strains in the plant rhizosphere by real-time PCR. [Appl Environ Microbiol. 2007]
Quantification of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens strains in the plant rhizosphere by real-time PCR.
Mavrodi OV, Mavrodi DV, Thomashow LS, Weller DM. Appl Environ Microbiol. 2007 Sep; 73(17):5531-8. Epub 2007 Jul 13.
Comparison of ATPase-encoding type III secretion system hrcN genes in biocontrol fluorescent Pseudomonads and in phytopathogenic proteobacteria. [Appl Environ Microbiol. 2004]
Comparison of ATPase-encoding type III secretion system hrcN genes in biocontrol fluorescent Pseudomonads and in phytopathogenic proteobacteria.
Rezzonico F, Défago G, Moënne-Loccoz Y. Appl Environ Microbiol. 2004 Sep; 70(9):5119-31.
Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. [Appl Environ Microbiol. 2003]
Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR.
Cupples AM, Spormann AM, McCarty PL. Appl Environ Microbiol. 2003 Feb; 69(2):953-9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Quantitative selective PCR of 16S ribosomal DNA correlates well with selective a...
Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots.
Appl Environ Microbiol. 1999 Apr ;65(4):1786-8.

PubMed
Production of poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) and poly(4-hy...
Production of poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) and poly(4-hydroxybutyric acid) without subsequent degradation by Hydrogenophaga pseudoflava.
Appl Environ Microbiol. 1999 Apr ;65(4):1570-7.

PubMed
[Psychosomatic pain in the field of gynecology. Stages of the formation of psych...
[Psychosomatic pain in the field of gynecology. Stages of the formation of psychosomatic reactions and symptoms with examples of cases (author's transl)].
Geburtshilfe Frauenheilkd. 1976 Dec ;36(12):1043-53.

PubMed
1H-NMR spectroscopy of body fluids: inborn errors of purine and pyrimidine metab...
1H-NMR spectroscopy of body fluids: inborn errors of purine and pyrimidine metabolism.
Clin Chem. 1999 Apr ;45(4):539-48.

PubMed
PGG-glucan, a soluble beta-(1,3)-glucan, enhances the oxidative burst response, ...
PGG-glucan, a soluble beta-(1,3)-glucan, enhances the oxidative burst response, microbicidal activity, and activates an NF-kappa B-like factor in human PMN: evidence for a glycosphingolipid beta-(1,3)-glucan receptor.
Immunopharmacology. 1999 Feb ;41(2):89-107.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: