Crystallization and preliminary X-ray analysis of ...[Acta Crystallogr D Biol Crystallogr. 1999]

SearchDatabase nameforSearch term

Advanced Search

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):699-701. Links

Crystallization and preliminary X-ray analysis of a new L-aminopeptidase-D-amidase/D-esterase activated by a Gly-Ser peptide bond hydrolysis.

Bompard-Gilles C, Villeret V, Fanuel L, Joris B, Frère JM, Van Beeumen J.

Laboratorium voor Eiwitbiochemie en Eiwitengineering, Universiteit Gent, K. L. Ledeganckstraat, 35, B-9000 Gent, Belgium.

Ochrobactrum anthropi possesses an L-aminopeptidase (DmpA) also able to act as a D-amidase/D-esterase. DmpA (40 kDa) is activated by auto-catalyzed protein splicing liberating an alpha-amino group presumably used as a general base in the catalytic mechanism. Two crystal forms were obtained at 294 K in 13-16% PEG 2000 mono-methylether at pH 9.0, adding either 0.2 M magnesium chloride or 1 M lithium chloride. Crystals of the first form belong to the space group C2221 and diffract to 3.0 A resolution, whereas crystals of the second form belong to the space group P21212 and diffract to 2.3 A resolution. Initial screening for heavy-atom derivatives on form II crystals, has led to a well substituted Hg derivative.

PMID: 10089474 [PubMed - indexed for MEDLINE]

A new variant of the Ntn hydrolase fold revealed by the crystal structure of L-aminopeptidase D-ala-esterase/amidase from Ochrobactrum anthropi.
Structure. 2000 Feb 15; 8(2):153-62.

[Structure. 2000]
A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for beta-alanyl dipeptides.
FEBS J. 2005 Jun; 272(12):3075-84.

[FEBS J. 2005]
Spontaneous resolution of binary copper(II) complexes with racemic dipeptides: crystal structures of glycyl-L-alpha-amino-n-butyrato copper(II) monohydrate, glycyl-D-valinato copper(II) hemihydrate, and glycyl-L-valinato copper(II) hemihydrate.
J Inorg Biochem. 2005 Aug; 99(8):1611-8.

[J Inorg Biochem. 2005]
Two new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide.
Cell Mol Life Sci. 1999 May; 55(5):812-8.

[Cell Mol Life Sci. 1999]
Inhibition of the aminopeptidase from Aeromonas proteolytica by L-leucinephosphonic acid. Spectroscopic and crystallographic characterization of the transition state of peptide hydrolysis.
Biochemistry. 2001 Jun 19; 40(24):7035-46.

[Biochemistry. 2001]
» See reviews... | » See all...

Patient Drug Information

Lithium (Eskalith CR® , Eskalith® , Lithobid® )
Lithium is used to treat and prevent episodes of mania (frenzied, abnormally excited mood) in people with bipolar disorder (manic depressive disorder; a disease that causes episodes of depression, episodes of mania, and ...
Docusate (Docusate calcium, Stool softener, Sulfolax, ...)
How do the drugs used to treat chronic constipation and irritable bowel syndrome with predominant constipation compare?
Source: AHFS Consumer Medication Information

Recent Activity

Clear Turn Off Turn On

Crystallization and preliminary X-ray analysis of a new L-aminopeptidase-D-amidase/D-ester...Crystallization and preliminary X-ray analysis of a new L-aminopeptidase-D-amidase/D-esterase activated by a Gly-Ser peptide bond hydrolysis.

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

Display Show

Crystallization and preliminary X-ray analysis of a new L-aminopeptidase-D-amidase/D-esterase activated by a Gly-Ser peptide bond hydrolysis.

Related articles

Patient Drug Information

Recent Activity