Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
J Orthop Trauma. 1999 Feb;13(2):102-6.

Efficacy of gentamycin-impregnated resorbable hydroxyapatite cement in treating osteomyelitis in a rat model.

Author information

  • 1Department of Orthopaedics and Rehabilitation, University of California-Davis, Sacramento 95817, USA.

Abstract

OBJECTIVE:

To test the effectiveness of a self-setting hydroxyapatite cement (HAC) as a carrier of gentamycin for the treatment of chronic osteomyelitis in a rat model by using a void-fill placement technique.

DESIGN:

Osteomyelitis of the tibia was created with Staphylococcus aureus (ATCC 49230) in sixty retired female breeder Sprague-Dawley rats by using the model by Korkusuz et al. (J Bone Joint Surg 1993;75B:111-114). At seven weeks after infection, all animals demonstrated clinical and radiographic signs of osteomyelitis and were debrided and divided into four treatment groups: A, debridement only; B, debridement and daily intraperitoneal gentamycin (0.2 milligram per kilogram per day); C, debridement and gentamycin-impregnated HAC in a void-fill model (1.0 milligram per kilogram of gentamycin); and D, debridement and gentamycin-impregnated polymethylmethacrylate (PMMA) beads (1.0 milligram per kilogram of gentamycin). Tibiae were harvested at zero weeks (control, n = 6), three weeks (n = 3 per group), five weeks (n = 4 per group), and seven weeks (n = 4 per group) and analyzed with quantitative bacteriologic analysis.

OUTCOME MEASUREMENT:

Qualitative bacteriologic analysis was performed by using serial dilution plating of homogenized tissue samples on standard soy trypticase agar plates. Reexamination by phage typing was performed to exclude contamination.

RESULTS:

The quantitative counts for Groups C (HAC) and D (PMMA) were significantly less (p < 0.003) than those for Group A (debridement alone) or Group B (intraperitoneal gentamycin) at all time points after time zero. There was no difference between Groups C and D at any time point.

CONCLUSION:

HAC is an effective adjuvant in treating chronic osteomyelitis in a rat model when using a void-fill placement technique.

PMID:
10052784
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Lippincott Williams & Wilkins
    Loading ...
    Write to the Help Desk