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Nat Biotechnol. 1999 Feb;17(2):149-55.

De novo reconstitution of a functional mammalian urinary bladder by tissue engineering.

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  • 1Department of Urology, Children's Hospital and Harvard Medical School, Boston, MA, USA.

Abstract

Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in culture from canine native bladder biopsies and seeded onto preformed bladder-shaped polymers. The native bladders were subsequently excised from canine donors and replaced with the tissue-engineered neo-organs. In functional evaluations for up to 11 months, the bladder neo-organs demonstrated a normal capacity to retain urine, normal elastic properties, and histologic architecture. This study demonstrates, for the first time, that successful reconstitution of an autonomous hollow organ is possible using tissue-engineering methods.

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PMID:
10052350
[PubMed - indexed for MEDLINE]
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