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J Chromatogr A. 1999 Jan 15;830(2):301-9.

Fast separation of (poly)phenolic compounds from apples and pears by high-performance liquid chromatography with diode-array detection.

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  • 1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá, Madrid, Spain.


Polyphenolic compounds in apples and pears were analysed by HPLC on C18-modified silica. Gradient elution with phosphoric acid-methanol mixtures and phosphoric acid-acetonitrile mixtures gave complete separation of all polyphenolics of interest. The use of methanol as modifier was preferred because it provides a more rapid separation (20 min). Diode-array detection was used for the provisional identification of polyphenolic compounds not available as standards.

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