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Nucleic Acids Res. 1999 Mar 15;27(6):1558-60.

Amplification of cDNA ends based on template-switching effect and step-out PCR.

Author information

  • 1Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117871, Russia.

Abstract

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

PMID:
10037822
[PubMed - indexed for MEDLINE]
PMCID:
PMC148354
Free PMC Article
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