Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents

H K Nguyen; O Fournier; U Asseline; D Dupret; N T Thuong

doi:10.1093/nar/27.6.1492

Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents

Nucleic Acids Res. 1999 Mar 15;27(6):1492-8. doi: 10.1093/nar/27.6.1492.

Authors

H K Nguyen¹, O Fournier, U Asseline, D Dupret, N T Thuong

Affiliation

¹ Centre de Biophysique Moléculaire, CNRS, Rue Charles Sadron, 45071 Orléans Cedex 02, France and Appligène-Oncor, Parc d'Innovation, Rue de Geiler de Kaysersberg, BP 72, 67402 Illkirch, France.

Abstract

The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA+along with the chemical modification of duplexes allow the fine adjustment of T m and the possibility of obtaining several duplex systems with varied isostabilizedtemperatures, some of which show greater stability than those of natural DNA. This approach could be very useful for DNA sequencing by hybridization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkynes / chemistry
Base Pairing / drug effects*
Cytosine / analogs & derivatives
Cytosine / chemistry
DNA / chemistry*
Hot Temperature
Nucleic Acid Denaturation
Nucleosides / chemistry*
Oligonucleotide Array Sequence Analysis
Quaternary Ammonium Compounds / pharmacology*
Uracil / analogs & derivatives
Uracil / chemistry

Substances

Alkynes
Nucleosides
Quaternary Ammonium Compounds
5-propynyluracil
Uracil
N(4)-methylcytosine
Cytosine
DNA