Strain: C57BL6 Pituitary, Male total RNA (MoPi.Male) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoPi.Male.sig22 Cell type Pituitary, Male Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoPi.Male_sig22.5253W.g-20 12/21/2004 458280 10125 QC Passed MoPi.Male_sig22.5253W.e-20 12/5/2004 316417 6378 QC Passed MoPi.Male_sig22.5253F.d-20 12/3/2004 396477 3789 QC Passed MoPi.Male_sig22.5253F.c-20 11/30/2004 367465 3747 QC Passed MoPi.Male_sig22.5253W.d-20 11/30/2004 251051 6468 QC Passed MoPi.Male_sig22.5253F.b-20 11/15/2004 270215 2094 QC Passed Run group: Total Beads successfully sequenced - 2059905 Processed Signatures - 10905
