Strain: C57BL6 Brain:Cerebellum, Male total RNA (MoBr.MaCb) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoBr.MaCb.sig21 Cell type Brain:Cerebellum, Male Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoBr.MaCb_sig21.5166W.d-20 10/5/2004 474748 15446 QC Passed MoBr.MaCb_sig21.5166W.c-20 10/2/2004 348203 12931 QC Passed MoBr.MaCb_sig21.5166F.c-20 10/1/2004 273331 10446 QC Passed MoBr.MaCb_sig21.5166F.a-20 9/17/2004 517363 23043 QC Passed MoBr.MaCb_sig21.5166F.b-20 9/17/2004 298009 12544 QC Passed MoBr.MaCb_sig21.5166W.b-20 9/16/2004 310871 12182 QC Passed Run group: Total Beads successfully sequenced - 2222525 Processed Signatures - 27979
