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| Status |
Public on Mar 29, 2016 |
| Title |
Expression data from Drosophila mutants´ (misexpression and overexpression) of cabut/dTIEG gene |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
cabut (cbt), is the Drosophila ortholog of the human TGF-β Inducible Early Gene 1 (dTIEG). It encodes a transcription factor involved in Drosophila dorsal closure (DC), and it is expressed in embryonic epithelial sheets and yolk cell during this process upon activation of the Jun N-terminal kinase (JNK) signaling pathway. Additional studies suggest that cbt may have a role in multiple developmental processes.
We used microarrays to detail the target genes of Cbt during dorsal closure process
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| Overall design |
cbtEP(2)2237E1 homozigote Drosophila mutant embryos were selected at 10-13 stage (during dorsal closure process) from non-GFP embryos from the line cbtEP(2)2237E1/Cy0, twi-eGFP line by embryo sorter assays. In parallel, samples of white embryos at same stages (as control) were sorting also by embryo sorter. Also using UAS-GAL4 system, Cbt was overexpressed in the embrionic epidermis during 10-13 stage by epidermal driver (69B-Gal4) and UAS-Cbt line.
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| Contributor(s) |
Belacortu Y, Paricio N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 28, 2016 |
| Last update date |
May 04, 2018 |
| Contact name |
YAIZA BELACORTU |
| E-mail(s) |
bepasyai@uv.es
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| Phone |
+34 652375801
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| Organization name |
UNIVERSITY OF VALENCIA
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| Department |
GENETICS
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| Lab |
MOLECULAR DEVELOPMENTAL GENETICS
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| Street address |
DR. MOLINER
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| City |
BURJASSOT |
| State/province |
VALENCIA |
| ZIP/Postal code |
46100 |
| Country |
Spain |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (9)
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| GSM2100406 |
cbtEP(2)2237E1 embryos at 10-13 stage, rep1 |
| GSM2100407 |
cbtEP(2)2237E1 embryos at 10-13 stage, rep2 |
| GSM2100408 |
cbtEP(2)2237E1 embryos at 10-13 stage, rep3 |
| GSM2100409 |
white 1118 embryos at 10-13 stage, control, rep1 |
| GSM2100410 |
white 1118 embryos at 10-13 stage, control, rep2 |
| GSM2100411 |
white 1118 embryos at 10-13 stage, control, rep3 |
| GSM2100412 |
Cbt>69B embryos at 10-13 stage, rep1 |
| GSM2100413 |
Cbt>69B embryos at 10-13 stage, rep2 |
| GSM2100414 |
Cbt>69B embryos at 10-13 stage, rep3 |
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| Relations |
| BioProject |
PRJNA316594 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79646_RAW.tar |
12.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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