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Series GSE75132 Query DataSets for GSE75132
Status Public on Jan 01, 2016
Title TMEM45A, SERPINB5 and p16INK4A transcript levels are predictive for development of high-grade cervical lesions
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Women persistently infected with human papillomavirus (HPV) type 16 are at high risk for development of cervical intraepithelial neoplasia grade 3 or cervical cancer (CIN3+). We aimed to identify biomarkers for progression to CIN3+ in women with persistent HPV16 infection. In this prospective study, 11,088 women aged 20–29 years were enrolled during 1991-1993, and re-invited for a second visit two years later. Cervical cytology samples obtained at both visits were tested for HPV DNA by Hybrid Capture 2 (HC2), and HC2-positive samples were genotyped by INNO-LiPA. The cohort was followed for up to 19 years via a national pathology register. To identify markers for progression to CIN3+, we performed microarray analysis on RNA extracted from cervical swabs of 30 women with persistent HPV16-infection and 11 HPV-negative women. After further validation, we found that high mRNA expression levels of TMEM45A, SERPINB5 and p16INK4a were associated with increased risk of CIN3+ in persistently HPV16-infected women.
 
Overall design We aimed at identifying genes differentially expressed in women with persistent HPV16 infection that either progressed to CIN3+ or not. As a test of principle we first compared HPV16 persistently infected women with HPV-negative women.
 
Contributor(s) Manawapat-Klopfer A, Russ R, Gmuender H, Stubenrauch F, Kjaer SK, Iftner T
Citation(s) 27508094
Submission date Nov 18, 2015
Last update date Mar 25, 2019
Contact name Frank Stubenrauch
E-mail(s) frank.stubenrauch@med.uni-tuebingen.de
Organization name University Hospital Tuebingen
Department Institute for Medical Virology
Lab Stubenrauch
Street address Elfriede-Aulhorn-Str. 6
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (41)
GSM1943705 Cervical swab_normal_rep1
GSM1943706 Cervical swab_normal_rep2
GSM1943707 Cervical swab_normal_rep3
Relations
BioProject PRJNA302609

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Supplementary file Size Download File type/resource
GSE75132_RAW.tar 177.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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