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| Status |
Public on Feb 14, 2007 |
| Title |
ENCODE ChIP-chip for Rel A (p65) using polyclonal antibody (N-) on TNF-alpha-stimulated HeLaS3 Cells [unpublished] |
| Project |
Pilot ENCODE
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| Organism(s) |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Rel A (p65) ChIP-chip using polyclonal antibody (N-terminus) performed on TNF-alpha-stimulated HeLaS3 cells for Nimblegen ENCODE arrays which comprise 50mer oligonucleotides spaces every 38bps (overlapping by 12nts). Goal was to identify p65-binding regions in the inflammatory and immune response of human cells.
Keywords: ChIP-chip
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| Overall design |
DNA from immunoprecipitated chromatin using p65 antibody (N-terminus) or normal rabbit IgG was hybridized to a Nimblegen ENCODE tiling array containing overlapping 50nt oligonucleotide probes. Samples are three biological replicates.
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| Web Link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
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| Contributor(s) |
Hong M, Zhong G, Rozowsky JS, Gerstein MB, Snyder MP |
| Submission date |
Jan 29, 2007 |
| Contact name |
Guoneng Zhong |
| E-mail(s) |
guoneng.zhong@yale.edu
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| Organization name |
Yale Med
|
| Street address |
300 George St
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL2533 |
Nimblegen ENCODE tiling array (HG17) with 50mer probe at 38bp interval for RenLab |
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| Samples (3) |
| GSM159256 |
p65 ChIP-chip on TNF-alpha-stimulated HeLaS3 cells using polyclonal antibody (N-) [unpublished] Sample 1 |
| GSM159257 |
p65 ChIP-chip on TNF-alpha-stimulated HeLaS3 cells using polyclonal antibody (N-) [unpublished] Sample 2 |
| GSM159258 |
p65 ChIP-chip on TNF-alpha-stimulated HeLaS3 cells using polyclonal antibody (N-) [unpublished] Sample 3 |
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