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| Status |
Public on Feb 14, 2007 |
| Title |
STAT1 (Interferon-alpha) ChIP-ChIP from HeLaS3 Cells using the NimbleGen ENCODE Array |
| Project |
Pilot ENCODE
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| Organism(s) |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
STAT1 ChIP DNA was isolated from HeLaS3 cells following Interferon-alpha stimulation for 30 min and from HeLaS3 cells left untreated. These two STAT1 ChIP DNA samples were combined and compared in the ENCODE regions on NimbleGen ENCODE arrays.
Keywords: ChIP-chip
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| Overall design |
Cultures of HeLaS3 cells were divided in half and were either stimulated with Interferon-alpha for 30 min. or left untreated. STAT1-ChIP DNA was isolated from both halves of the culture, labeled and then combined and hybridized to a NimbleGen ENCODE tiling array that contains overlapping 50nt oligonucleotide probes. The series is comprised of three biological replicates, hybridized separately to three arrays.
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| Web Link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
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| Contributor(s) |
Euskirchen G, Zhong G, Rozowsky JS, Gerstein MB, Snyder MP |
| Submission date |
Jan 29, 2007 |
| Contact name |
Guoneng Zhong |
| E-mail(s) |
guoneng.zhong@yale.edu
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| Organization name |
Yale Med
|
| Street address |
300 George St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL2533 |
Nimblegen ENCODE tiling array (HG17) with 50mer probe at 38bp interval for RenLab |
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| Samples (3) |
| GSM159174 |
STAT1 (Interferon-alpha) ChIP-ChIP from HeLaS3 applied to the NimbleGen ENCODE Array Sample 1 [unpublished] |
| GSM159175 |
STAT1 (Interferon-alpha) ChIP-ChIP from HeLaS3 applied to the NimbleGen ENCODE Array Sample 2 [unpublished] |
| GSM159176 |
STAT1 (Interferon-alpha) ChIP-ChIP from HeLaS3 applied to the NimbleGen ENCODE Array Sample 3 [unpublished] |
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