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| Status |
Public on Nov 30, 2006 |
| Title |
ENCODE ChIP-chip for RNA Polymerase II using monoclonal antibody (8WG16) on HeLaS3 Cells [unpublished] |
| Project |
Pilot ENCODE
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| Organism(s) |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
RNA Polymerase II ChIP-chip using monoclonal antibody (8WG16) performed on HeLaS3 Cells for Nimblegen ENCODE arrays which comprise 50mer oligonucleotides spaces every 38bps (overlapping by 12nts). Goal was to identify Pol II-binding regions. Use of this data requires permission from its producers.
Keywords: ChIP-chip
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| Overall design |
DNA from immunoprecipitated chromatin using Pol II antibody (8WG16) or normal rabbit IgG was hybridized to a Nimblegen ENCODE tiling array containing overlapping 50nt oligonucleotide probes. Samples are three biological replicates.
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| Web Link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
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| Contributor(s) |
Hong M, Zhong G, Rozowsky JS, Gerstein MB, Snyder MP |
| Submission date |
Nov 29, 2006 |
| Contact name |
Guoneng Zhong |
| E-mail(s) |
guoneng.zhong@yale.edu
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| Organization name |
Yale Med
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| Street address |
300 George St
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
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| Samples (3) |
| GSM147436 |
RNA Polymerase II ChIP-chip on HeLaS3 cells using monoclonal antibody (8WG16) Sample 1 |
| GSM147437 |
RNA Polymerase II ChIP-chip on HeLaS3 cells using monoclonal antibody (8WG16) Sample 2 |
| GSM147438 |
RNA Polymerase II ChIP-chip on HeLaS3 cells using monoclonal antibody (8WG16) Sample 3 |
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