|
| Status |
Public on Jun 11, 2014 |
| Title |
Linking Notch signaling to ischemic stroke |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Using a hitherto uncharacterized knockout mouse model of Notch 3, a Notch signaling receptor paralogue highly expressed in vascular SMCs, we uncover a striking susceptibility to ischemic stroke upon challenge. Cellular and molecular analyses of vascular SMCs derived from these animals associate Notch 3 activity to the expression of specific gene targets, whereas genetic rescue experiments unambiguously link Notch 3 function in vessels to the ischemic phenotype.
|
| |
|
| Overall design |
Microarray Studies. Biotinylated cRNA samples from freshly sorted brain SMCs (four Notch 3 +/− mice and five Notch 3 −/− mice) were fragmented before hybridization (15 μg each) onto mouse 430 2.0 Affymetrix chips. The chips were washed, stained by using strepavidin-phycoerytrin, and scanned the next day as described in ref. 24. For data normalization, all probe sets were scaled to a target intensity of 150. Microarray data analysis was performed by using Rosetta Resolver. All cells were from 10- to 12-week-old male mice. Gene Ontology Analyses. PANTHER software was used to define over- and underrepresented functions in the list of signature genes found by microarray analysis (25). P values were calculated by using binomial statistics.
|
| |
|
| Contributor(s) |
Arboleda-Velasquez J, Artavanis-Tsakonas S |
| Citation(s) |
25015359 |
| Submission date |
Jun 10, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Joseph Arboleda-Velasquez |
| E-mail(s) |
vincent_primo@meei.harvard.edu
|
| Phone |
6179122558
|
| Organization name |
Schepens Eye Research Institute
|
| Department |
Opthalmology
|
| Lab |
D'Amore lab
|
| Street address |
20 Staniford st
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
01906 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA252379 |
Less...
More...