|
| Status |
Public on Sep 01, 2006 |
| Title |
Improvements in sample preparation for ChIP-chip assays |
| Project |
Pilot ENCODE
|
| Organism(s) |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
We have improved the new protocol for ChIP-chip by using pooling method. The new method has produced reproducible binding patterns and low background signals.
Keywords: ChIP-chip
|
| |
|
| Overall design |
We have performed and compared three methods for ChIP-chip samples: LMPCR, 10 Pooled Samples and WGA.
|
| Web Link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
|
| |
|
| Contributor(s) |
O'Geen H, Nicolet CM, Blahnik K, Green R, Farnham PJ |
| Citation(s) |
17140114
|
| Submission date |
Jul 06, 2006 |
| Contact name |
Victor X Jin |
| E-mail(s) |
vxjin@ucdavis.edu
|
| Phone |
530-7549729
|
| Organization name |
UC Davis
|
| Department |
Genome Center
|
| Lab |
Peggy J. Farnham
|
| Street address |
One Shields Ave. GBSF 4502
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3980 |
UC Davis Human ENCODE array |
|
| Samples (6)
|
| GSM118662 |
Human Ntera2 cells, Oct4 ENCODE Sample D |
| GSM118663 |
Human Ntera2 cells, Oct4 ENCODE Sample N |
| GSM118699 |
Human Ntera2 cells, Oct4 ENCODE Sample E1 |
| GSM118700 |
Human Ntera2 cells, Oct4 ENCODE Sample G |
| GSM118701 |
Human Ntera2 cells, Oct4 ENCODE Sample H |
| GSM118704 |
Human Ntera2 cells, Oct4 ENCODE Sample K |
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