|
| Status |
Public on Oct 31, 2013 |
| Title |
Deep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaena sp. strain PCC 7120 |
| Organism |
Nostoc sp. PCC 7120 = FACHB-418 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We employed chromatin pull-down and deep sequencing to globally identify HetR DNA targets in vivo at 6 hours after fixed-nitrogen deprivation. We identified novel DNA binding targets of tagged HetR-6xHis and defined a consensus HetR binding site from these HetR target sequences.
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| |
|
| Overall design |
Chromatin pull down of hetR mutant strain UHM103 carrying pAM4375, which expresses HetR-6xHis, and a wild-type control. One dataset was collected.
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| |
|
| Contributor(s) |
Golden JW, Flaherty BL |
| Citation(s) |
25278209 |
| |
| Submission date |
Oct 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
James W Golden |
| E-mail(s) |
jwgolden@ucsd.edu
|
| Phone |
858-246-0643
|
| Organization name |
UCSD
|
| Department |
Biology
|
| Lab |
James Golden
|
| Street address |
9500 Gilman Dr # 0116
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0116 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17863 |
Illumina HiSeq 1000 (Nostoc sp. PCC 7120) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA225677 |
| SRA |
SRP032373 |
