|
| Status |
Public on May 04, 2006 |
| Title |
Murine intestinal lymphoid tissue infected with Yersinia enterocolitica |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To gain a comprehensive view of the host response to pathogens within these tissues, we determined the transcriptional profiles of intestinal lymphatic tissue infected with Y. enterocolitica. Expression analysis using Affymetrix GeneChips revealed a complex host response in the Peyer’s patches (PP) and mesenteric lymph nodes (MLN) following oral infection with Y. enterocolitica.
Keywords: Disease state analysis
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| |
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| Overall design |
Peyer's patches and mesenteric lymph nodes were surgically removed from uninfected (control) and infected (experimental) mice at 12 hours, day 2, 4 and 7 post oral infection. Tissue samples from 10 mice per time-point were combined. Total RNA was extracted using the Trizol method prior to total cDNA syntesis, labeling and hybridization to Affymetrix MGU74Av2 GeneChips. Data was processed and analyzed using MAS 5.0 and custom analysis protocols. Samples from Peyer's patches and mesenteric lymph nodes are included in this series.
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| |
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| Contributor(s) |
Handley SA, Dube PH, Miller VL |
| Citation(s) |
16717182 |
| Submission date |
May 02, 2006 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Scott Allyn Handley |
| E-mail(s) |
handley.scott@gmail.com
|
| Organization name |
Washington University
|
| Department |
Molecular Microbiology
|
| Lab |
Dr. Virginia Miller
|
| Street address |
660 South Euclid Avenue
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (15)
|
| GSM107566 |
C57BL/6j Peyer's patches_Uninfected_01 |
| GSM107568 |
C57BL/6j Peyer's patches_Uninfected_02 |
| GSM107569 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_12 Hours |
| GSM107570 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_12 hours_02 |
| GSM107571 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_Day 2_01 |
| GSM107572 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_Day 2_02 |
| GSM107573 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_Day 4_01 |
| GSM107574 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_Day 4_02 |
| GSM107575 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_Day 7_01 |
| GSM107576 |
C57BL/6j Peyer's patches_Infected_Y.enterocolitica_Day 7_02 |
| GSM107665 |
C57BL/6j mesenteric lymph nodes_uninfected |
| GSM107666 |
C57BL/6j mesenteric lymph nodes_infected_Y.enterocolitica_12 hour |
| GSM107667 |
C57BL/6j mesenteric lymph nodes_infected_Y.enterocolitica_2 days |
| GSM107668 |
C57BL/6j mesenteric lymph nodes_infected_Y.enterocolitica_day 4 |
| GSM107669 |
C57BL/6j mesenteric lymph nodes_infected_Y.enterocolitica_day 7 |
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| Relations |
| BioProject |
PRJNA95581 |
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