|
| Status |
Public on Jun 15, 2013 |
| Title |
Genome-wide methylation and expression analysis of two breast cancer cell lines [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To improve our understanding of the relationships between methylation and expression we profiled mRNA expression and single-base resolution methylation levels for two breast cancer cell lines, MCF7 and T47D. Expression was profiled using RNA-seq. Methylation was assayed using Methyl-MAPS, which uses methylation-sensitive and -dependent restriction enzyme digests followed by high-throughput sequencing to identify methylation levels at individual CpGs (Edwards et al. 2010, Genome Research).
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| |
|
| Overall design |
RNA-Seq was used to generate mRNA expression profiles of MCF7 and T47D cells under standard growth conditions.
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| |
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| Contributor(s) |
VanderKraats ND, Edwards JR, Hiken JF, Decker KF |
| Citation(s) |
23748561, 27869171 |
| Submission date |
Mar 20, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Nathan VanderKraats |
| E-mail(s) |
ndvk1980@yahoo.com
|
| Phone |
314-362-2348
|
| Organization name |
Washington University in St. Louis, School of Medicine
|
| Department |
Center for Pharmacogenomics
|
| Lab |
Edwards
|
| Street address |
660 S. Euclid Ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE45337 |
Genome-wide methylation and expression analysis of two breast cancer cell lines |
|
| Relations |
| BioProject |
PRJNA193492 |
| SRA |
SRP019817 |
