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| Status |
Public on Dec 01, 2013 |
| Title |
Rickettsia rickettsii transcriptome - Blood feeding response |
| Organism |
Rickettsia rickettsii |
| Experiment type |
Expression profiling by array
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| Summary |
One of the most important vectors of the Brazilian Spotted Fever, the tick Amblyomma aureolatum in Brazil was used in this study. We laboratorial controlled the infection of adult females of A. aureolatum with the virulent brazilian strain Taiacu of Rickettsia rickettsii. The group of ticks was divided into 2 testing groups, group 2 (G2) composed of adult females incubated at 35°C for 3 days that were not fed after molting to adults and group 3 (G3) composed of adult females fed on its favorite natural host, the dog (Canis familiaris) also for 3 days. Right after incubation or feeding, ticks were individually dissected and all internal organs were transferred to RNAlater® Solution (Life Technologies) until gDNA and total RNA simultaneously isolation. A total of 14 ticks of each group were analyzed in two biological replicates (7 ticks each). Dye-swap was also applied to construct the technical replicate of each biological sample
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| Overall design |
total RNA from both experimental samples (G2 and G3) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental group.
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| Contributor(s) |
Galletti MF, Fujita A, Malossi C, Labruna MB, Pinter A, Soares JF, Nishiyama-Jr MY, Daffre S, Fogaça AC |
| Citation(s) |
24155949 |
| Submission date |
Feb 14, 2013 |
| Last update date |
Mar 06, 2014 |
| Contact name |
Maria Fernanda Galletti |
| E-mail(s) |
mafegalletti@gmail.com
|
| Organization name |
Universidade de São Paulo - Instituto de Ciências Biomédicas - Departamento de Parasitologia
|
| Department |
Department of Parasitology
|
| Lab |
Bioquemistry and Immunology of Arthropods
|
| Street address |
Av. Prof. Lineu Prestes, 1374 sala 39
|
| City |
São Paulo |
| State/province |
São Paulo |
| ZIP/Postal code |
05508-900 |
| Country |
Brazil |
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| Platforms (1) |
| GPL16681 |
Agilent-024503 Rickettsia rickettsii str. Taiacu 15k v1.0 |
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| Samples (4)
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| GSM1082980 |
Rickettsia rickettsii transcriptome - ch2: G3 replicate 1; ch1: G2 replicate 1 |
| GSM1082981 |
Rickettsia rickettsii transcriptome - ch2: G2 replicate 1; ch1: G3 replicate 1 |
| GSM1082982 |
Rickettsia rickettsii transcriptome - ch2: G3 replicate 2; ch1: G2 replicate 2 |
| GSM1082983 |
Rickettsia rickettsii transcriptome - ch2: G2 replicate 2; ch1: G3 replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE44349 |
Natural blood feeding and temperature shift modulate the global transcriptional profile of Rickettsia rickettsii. |
|
| Relations |
| BioProject |
PRJNA189711 |
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