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Series GSE36999 Query DataSets for GSE36999
Status Public on Apr 16, 2012
Title Chip-chip from WT and Polycomb Component Knock Out Mouse ES cells for H2AZ, H3K27me3, EZH2 and Ring1B.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary The essential histone variant H2A.Z localises to both active and silent chromatin sites. In embryonic stem cells (ESCs), H2A.Z is also reported to co-localise with polycomb repressive complex 2 (PRC2) at developmentally silenced genes. The mechanism of H2A.Z targeting is not clear, but a role for the PRC2 component Suz12 has been suggested. Given this association, we wished to determine if polycomb functionally directs H2A.Z incorporation in ESCs. We demonstrate that the PRC1 component Ring1B interacts with multiple complexes in ESCs. Moreover, we show that although the genomic distribution of H2A.Z co-localises with PRC2, Ring1B and with the presence of CpG islands, H2A.Z still blankets polycomb target loci in the absence of Suz12, Eed (PRC2) or Ring1B (PRC1). Therefore we conclude that H2A.Z accumulates at developmentally silenced genes in ESCs in a polycomb independent manner.
 
Overall design Array design includes 2 biological replicates for all samples and technical replication (dye swaps for H3K27me3_WT_ES, EZH2_WT_ES, EZH2_RING1b_KO_ES, H2AZ_WT_ES(2), H2AZ_Eed_KO_ES and H2AZ_Suz12_KO_ES). Ring1B_WT_ES is represented by a single replicate.
 
Contributor(s) Illingworth R, Botting C, Grimes G, Bickmore WA, Eskeland R
Citation(s) 22496869
Submission date Apr 03, 2012
Last update date Dec 17, 2012
Contact name Rob S Illingworth
E-mail(s) robert.illingworth@ed.ac.uk
Phone 01316519640
Organization name The University of Edinburgh
Department Centre for regenerative Medicine
Lab Illingworth
Street address Centre for Regenerative Medicine
City Edinburgh
State/province Midlothian
ZIP/Postal code EH16 4UU
Country United Kingdom
 
Platforms (1)
GPL14936 NimbleGen_mouse(MM9)_Hox plus array [Design_ID:11926]
Samples (10)
GSM908268 H3K27me3 ChIP in WT ES Cells using anti H3K27me3 antibody (Millipore, 07-449)
GSM908269 H3K27me3 ChIP in Ring1B deficient ES Cells using anti H3K27me3 antibody (Millipore, 07-449)
GSM908270 EZH2 ChIP in WT ES Cells using anti EZH2 antibody (Millipore 07-689)
Relations
BioProject PRJNA157521

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36999_RAW.tar 637.6 Mb (http)(custom) TAR (of PAIR)
GSE36999_readme.txt.gz 842 b (ftp)(http) TXT
Processed data included within Sample table

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