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| Status |
Public on Apr 16, 2013 |
| Title |
Identification of differential expressed genes between P-RPCs, ESC-RPCs and Dkk1 treated ESC-RPCs through genome-wide transcript profiling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Tumor formation constitutes a major obstacle to the clinical application of embryonic stem cells (ESCs). As P-RPCs could successfully integrate into host eyes without development of teratomas or NOG, we sought to identify differentially expressed genes between P-RPCs and ESC-RPCs through genome-wide transcript profiling. Inhibition of Wnt signaling by DKK1 promotes the commitment of ESC-RPCs to more mature retinal cells and reduces the occurrence of NOG to 3%. DKK1-treated ESC-RPCs efficiently integrate to the host retina, form synaptic connections and restore visual function.
Here, we report that further differentiation of ESC-derived neural progenitors into retinal progenitor cells (ESC-RPCs) completely eliminates teratomas in ocular transplantation. However, tumor-like neural overgrowth (NOG) occurs in 61% of transplanted eyes.
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| Overall design |
ESC-RPCs were divided into two groups according to the differentiation stages for RNA extraction and hybridization on Affymetrix microarrays. Normal control ESC-RPCs (N) were represented the homogeneous populations of early stage expression profiles of immature ESC-RPCs. DKK1 treated ESC-RPCs (D) were represented the homogeneous populations of late stage expression profiles of further differetiated mature ESC-RPCs. To sought the pathways involved in the proliferation and oncogenesis of ESC-RPCs, the newborn C57 mice reitinal progenitor cells (R) were applied as negative control. Each group above had three independent biological repeats.
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| Contributor(s) |
Cui L, Guan Y |
| Citation(s) |
23524971 |
| Submission date |
Nov 29, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Lu Cui |
| E-mail(s) |
lcui@sibs.ac.cn
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| Organization name |
Institue of Health Sciences
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| Street address |
225 South Chongqing road
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| GSM840281 |
DKK1-treated ESC-RPCs, biological rep1 |
| GSM840282 |
DKK1-treated ESC-RPCs, biological rep2 |
| GSM840283 |
DKK1-treated ESC-RPCs, biological rep3 |
| GSM840284 |
Normal control ESC-RPCs, biological rep1 |
| GSM840285 |
Normal control ESC-RPCs, biological rep2 |
| GSM840286 |
Normal control ESC-RPCs, biological rep3 |
| GSM840287 |
Primary RPCs, biological rep1 |
| GSM840288 |
Primary RPCs, biological rep2 |
| GSM840289 |
Primary RPCs, biological rep3 |
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| Relations |
| BioProject |
PRJNA150579 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34002_RAW.tar |
32.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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