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| Status |
Public on Feb 26, 2002 |
| Title |
E. coli response to MMC |
| Organism(s) |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
Changes in gene expression after treatment of E. coli cultures with mitomycin C were assessed using gene array technology. Unexpectedly, a large number of genes (nearly 30% of all genes) displayed significant changes in their expression level. Analysis and classification of expression profiles of the corresponding genes allowed us to assign this large number of genes into a dozen to two dozen small clusters of genes with similar expression profiles. This assignment allowed us to describe systematically the changes in the level of gene expression in response to DNA damage. Among the damage-induced genes more than a hundred are novel. From those genes involved in DNA metabolism that have not been previously shown to be induced by DNA damage, for example, the mutS gene involved in mismatch repair is especially noteworthy. In addition to the SOS response, we observed the induction of other stress response pathways such as those of oxidative stress and osmotic protection. Among the genes that are down-regulated in response to DNA damage are numerous protein biosynthesis genes. Analysis of the gene expression data highlighted the essential involvement of sigmaS regulated genes and the general stress response network in the response to DNA damage.
Keywords = DNA damage, recA, SOS
Keywords: other
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| Contributor(s) |
Khil PP, Camerini-Otero R |
| Citation(s) |
11967071
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| Submission date |
Feb 25, 2002 |
| Contact name |
Natalya A Smirnova |
| E-mail(s) |
natalyas@intra.niddk.nih.gov
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| Phone |
(301)-496-4053
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| Fax |
(301)-496-9878
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| Organization name |
NIDDK
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| Department |
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| Lab |
Genetics and Biochemistry Branch
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| Street address |
5 Memorial Dr.
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL102 |
E. coli Panorama Gene Arrays |
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| Samples (16)
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