|
| Status |
Public on Sep 02, 2011 |
| Title |
Genome-wide maps of histone modifications unwind in vivo chromatin states of the hair follicle lineage |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mouse hair follicles (HFs) undergo synchronized cycles. Cyclical regeneration and hair growth is fueled by stem cells (SCs). During the rest phase, the HF-SCs remain quiescent due to extrinsic inhibitory signals within the niche. As activating cues accumulate, HF-SCs become activated, proliferate, and grow downward to form transient-amplifying matrix progenitor cells. We used microarrays to detect the relative levels of global gene expression underlying the states of hair follicle stem cells and their transient-amplifying progeny before differentiation.
|
| |
|
| Overall design |
Quiescent hair follicle stem cells (qHF-SCs), activated hair follicle stem cells (aHF-SCs) and transient-amplifying matrix cells (HF-TACs) were FACS-purified for RNA extraction and hybridization on Affymetrix microarrays. To obtain homogeneous populations of expression profiles, we applied the FACS technique to purify SC and TACs according to their cell surface markers.
|
| |
|
| Contributor(s) |
Lien W, Fuchs E |
| Citation(s) |
21885018 |
| Submission date |
Jul 28, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Wen-Hui Lien |
| E-mail(s) |
wlien@rockefeller.edu
|
| Phone |
212-327-7482
|
| Organization name |
The Rockefeller Unoversity
|
| Department |
Mammalian cell biology and development
|
| Lab |
Elaine Fuchs
|
| Street address |
1230 York Ave Box#300
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
| GSM768826 |
Quiescent hair follicle stem cells, rep1 |
| GSM768827 |
Quiescent hair follicle stem cells, rep2 |
| GSM768828 |
Activated hair follicle stem cells, rep1 |
| GSM768829 |
Activated hair follicle stem cells, rep2 |
| GSM768830 |
Transient-amplifying matrix cells, rep1 |
| GSM768831 |
Transient-amplifying matrix cells, rep2 |
|
| Relations |
| BioProject |
PRJNA144895 |
Less...
More...