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| Status |
Public on Dec 17, 2009 |
| Title |
Gene expression data from small intestines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Rb and E2F are thought to play antagonistic roles in celll proliferation. However, this model is based mostly from in vitro cell culture systems. We used small intestines to test this model in vivo.
We found that deletion of E2f1-3 in the small intestine of mice suppressed the ectopic expression of E2F targets and cell proliferation caused by Rb-deficiency. Surprisingly, E2f1-3 deletion failed to arrest the proliferation of intestinal cells containing an intact Rb gene, and instead led to E2F target derepression and apoptosis.
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| |
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| Overall design |
Total RNA of crypts and villi from wild-type, Rb-/-, E2f1-/-, E2f2-/-, E2f3-/-, E2f1-/-, E2f2-/-, and E2f3-/- small intestines. Small intestines were harvested 7 days after mice were injected intraperitoneally with beta-napthoflavone.
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| |
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| Contributor(s) |
Leone G, Pipas J |
| Citation(s) |
20016602 |
| Submission date |
Jun 05, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Gustavo Leone |
| E-mail(s) |
jean-leon.chong@osumc.edu
|
| Organization name |
The Ohio State University
|
| Department |
840A Biomedical Research Tower
|
| Street address |
640 west 12th avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (24)
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| Relations |
| BioProject |
PRJNA116023 |
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