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| Status |
Public on Feb 05, 2009 |
| Title |
Ago2 or Dicer knockdown effects on mRNA levels in HeLa |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of mRNA changes in HeLa cells following Ago2 or Dicer depletion. Dicer, a cytoplasmic RNase III, generates the mature form of small RNAs including microRNA. Ago2 is a component of an effector complex of microRNA.
Keywords: gene expression array-based (RNA / in situ oligonucleotide)
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| Overall design |
siRNA against Ago2 or Dicer were trasnfected into HeLa cells. siRNA against GFP was used as a control. Biologically duplicated total RNAs were prepared from HeLa cells, 24 hrs and 48 hrs after siRNA transfection.
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| Contributor(s) |
Han J, Kim Y, Kim VN |
| Citation(s) |
19135890 |
| Submission date |
Nov 18, 2008 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Jinju Han |
| Organization name |
Seoul National University
|
| Lab |
Bd. 504, Rm 501
|
| Street address |
599 Gwanangno, Gwanak-gu
|
| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE13640 |
Effect of miRNA biogenesis factors on mRNA levels |
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| Relations |
| BioProject |
PRJNA113875 |
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