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| Status |
Public on Aug 28, 2009 |
| Title |
Alterations of gene expression in human peripheral lymphocytes after exposure to cigarette smoke condensate in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about alteration of the global gene expression by cigarette smoke (CS) and few biomarkers for smoking-related harm are available.
We used Affymetrix HG-U133A GeneChips to measure the transcriptomes in eight cultured lymphocyte samples exposed to cigarette smoke condensate (CSC) in vitro . The in vitro exposure of lymphocytes to CSC significantly changed expression levels of 2,266 genes many of which biologically interacted. They included genes encoding for xenobiotic metabolism and oxidative stress-response (e.g. Nrf2 and AhR signaling pathways), inflammation/immune response (e.g. cytokines), apoptosis, cell cycle and tumorigenesis. However, the magnitude of expression responses for some genes showed high inter-individual variability.
Keywords: toxic effects on gene expression
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| Overall design |
The goals of this study were to evaluate novel gene expression profiles and pathways affected by cigarette smoke condensate (CSC), and to identify potential biomarkers for cigarette smoke exposure and harm. To this end, we isolated the PBMC from eight light smokers, cultured the cells in vitro and exposed them to 2R4F CSC, then determined the gene expression profiles with Affymetrix microarray and analyzed alteration of global gene expression after exposure to CSC.
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| Contributor(s) |
Chen J, Shields PG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 27, 2008 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Jinguo Chen |
| E-mail(s) |
jinguochen@yahoo.com
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| Organization name |
Georgetown University Lombardi Cancer Center
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| Department |
Cancer Genetics and Epidemiology
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| Street address |
3800 Reservoir Road NW
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| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20057 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE12587 |
Alterations of global gene expression by cigarette smoke |
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| Relations |
| BioProject |
PRJNA114231 |
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