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| Status |
Public on Aug 12, 2008 |
| Title |
Expression data from Arabidopsis seed compartments at the pre-globular stage |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected pre-globular stage seed compartments from 7-micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments of seeds containing pre-globular-stage embryos was identified as those seeds containing embryo propers made up of between 2 and 8 cells. For the purposes of this study we captured 6 compartments: embyro proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and general seed coat. Serial sections of entire seeds were also captured for comparison.
Keywords: cell type comparison
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| Overall design |
Pre-globular stage seed compartments or whole seeds were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, general seed coat, chalazal seed coat, whole seeds, 2 biological replicates each).
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| Contributor(s) |
Stone SL, Pelletier J, Kirkbride RC, Lu XH, Belmonte MF, Fei J, Cheng C, Golberg RB, Harada JJ |
| Citation(s) |
23319655, 20385809 |
| Submission date |
Aug 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
John J Harada |
| E-mail(s) |
jjharada@ucdavis.edu
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| Phone |
530-752-0673
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| Organization name |
University of California, Davis
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| Department |
Plant Biology
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| Lab |
Harada
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (14)
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| GSM311273 |
Laser capture microdissected (LCM) embryo proper at the pre-globular stage, biological replicate 1 |
| GSM311274 |
Laser capture microdissected (LCM) embryo proper at the pre-globular stage, biological replicate 2 |
| GSM311275 |
Laser capture microdissected (LCM) micropylar endosperm at the pre-globular stage, biological replicate 1 |
| GSM311276 |
Laser capture microdissected (LCM) micropylar endospermr at the pre-globular stage, biological replicate 2 |
| GSM311277 |
Laser capture microdissected (LCM) peripheral endosperm at the pre-globular stage, biological replicate 1 |
| GSM311278 |
Laser capture microdissected (LCM) peripheral endosperm at the pre-globular stage, biological replicate 2 |
| GSM311279 |
Laser capture microdissected (LCM) chalazal endosperm at the pre-globular stage, biological replicate 1 |
| GSM311280 |
Laser capture microdissected (LCM) chalazal endosperm at the pre-globular stage, biological replicate 2 |
| GSM311281 |
Laser capture microdissected (LCM) chalazal seed coat at the pre-globular stage, biological replicate 1 |
| GSM311282 |
Laser capture microdissected (LCM) chalazal seed coat at the pre-globular stage, biological replicate 2 |
| GSM311283 |
Laser capture microdissected (LCM) general seed coat at the pre-globular stage, biological replicate 1 |
| GSM311284 |
Laser capture microdissected (LCM) general seed coat at the pre-globular stage, biological replicate 2 |
| GSM311285 |
Laser capture microdissected (LCM) whole seed sections at the pre-globular stage, biological replicate 1 |
| GSM311286 |
Laser capture microdissected (LCM) whole seed sections at the pre-globular stage, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE12404 |
Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development |
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| Relations |
| BioProject |
PRJNA114185 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12402_RAW.tar |
27.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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