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Series GSE12215 Query DataSets for GSE12215
Status Public on Jul 29, 2008
Title Identification of transformation-related pathways in a breast epithelial cell model using a ribonomics approach.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Employing MCT-1 oncogene mediated transformation of immortalized breast epithelial MCF10A cells; we characterized the largely reciprocal association of these two RBPs with target mRNAs and their influence on protein expression vis-à-vis cellular transformation. Using a ribonomics approach, we identified mRNAs from cancer-related pathways whose association with AUF1 and/or HuR were altered when comparing immortalized with transformed MCF10A cells.
Significantly, we were able to demonstrate that knockdown of HuR expression using RNA interference, reduced anchorage-independent growth capacity in transformed MCF10A cells as well as decreased protein expression of a number of validated target genes. Our data demonstrate that the global alterations in binding of HuR and AUF1 with target transcripts have a critical role in post-transcriptional regulation of genes encoding proteins involved in breast epithelial cell transformation.
 
Overall design In this study, using a microarray approach we demonstrate that the dynamic global changes in association of two RBPs, HuR and AUF1, with cancer-related mRNAs have important influence on cell transformation in a MCT-1-mediated breast epithelial transformation model.
 
Contributor(s) Mazan-Mamczarz K, Hagner PR, Dai B, Wood III WH, Zhang Y, Becker KG, Liu Z, Gartenhaus RB
Citation(s) 18829526
Submission date Jul 23, 2008
Last update date Jun 22, 2020
Contact name Supriyo De
Organization name NIA-IRP, NIH
Department Laboratory of Genetics and Genomics
Lab Computational Biology & Genomics Core
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
 
Platforms (1)
GPL6102 Illumina human-6 v2.0 expression beadchip
Samples (12)
GSM307174 pLXSN/AUF1/1
GSM307175 pLXSN/AUF1/2
GSM307176 pLXSN/AUF1/3
Relations
BioProject PRJNA113731

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12215.txt 10.0 Mb (ftp)(http) TXT
GSE12215_RAW.tar 5.8 Mb (http)(custom) TAR
Processed data included within Sample table

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