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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 17, 2009 |
| Title |
ligand-activated and -unactivated AHR in wiltype and mutant hepatoma cells |
| Organism(s) |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Starting with our early global expression analyses of TCDD-treated human hepatoma cells {Puga, 2000 4679 /id}, the AHR transcriptional induction profile has been extensively studied, whether activated by TCDD, B[a]P or in the absence of exogenous ligands (reviewed in {Frericks, 2007 5618 /id}). In addition to using prior knowledge to integrate expression profiles into the AHR gene target network, we performed a new set of expression profile analyses of wild type Hepa-1c1c7 and c35 cell lines and compared the responses in naïve cells with responses in TCDD or B[a]P exposed cells for 8 hours. Results of our expression array studies are in close agreement with current knowledge.
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| Overall design |
Three biological replicates were each performed for wildype naïve cells (DMSO), TCDD activated cells, and B[a]P activated cells, and for c35 (AHR mutant) naïve cells (DMSO), TCDD activated cells, and B[a]P activated cells.
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| Contributor(s) |
Sartor MA, Schnekenburger M, Marlowe JL, Reichard JF, Wang Y, Fan Y, Ma C, Karyala S, Hableib D, Liu X, Medvedovic M, Puga A |
| Citation(s) |
19654925
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| Submission date |
Jun 16, 2008 |
| Contact name |
Maureen A Sartor |
| Organization name |
University of Michigan
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| Street address |
2017 Palmer Commons-100 Washtenaw Ave.
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2218 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (18)
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE11796_RAW.tar |
65.2 Mb |
(ftp)(http) |
TAR (of CEL) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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