Overgo probe consists of two 24mer oligonucleotides, derived from genomic sequence, which share eight base pairs of complimentary sequence at their 3' ends. After synthesis and annealing, they create a sixteen base overhang. This overhang is "filled in " using Klenow fragment thereby incorporating appropriate radionucleotides. Typically, 32P-dATP and 32P-dCTP are used and the GC-content of the oligonucleotides is kept between 40-60%. The resulting product is a double-stranded 40mer with, on average, eight radiolabeled nucleotides incorporated per single-stranded product. This probe is quite sequence specific with high specific activity (40-70% 32-P dNTP incorporation rate). Overgo probes are used for genome mapping.
» About Overgo technology in PubMed
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