In Situ Hybridization (ISH)
In Situ Hybridization (ISH) is a technique that allows for precise localization of a specific segment of nucleic acid within a histologic section. The underlying basis of ISH is that nucleic acids, if preserved adequately within a histologic specimen, can be detected through the application of a complementary strand of nucleic acid to which a reporter molecule is attached.
Visualization of the reporter molecule allows to localize DNA or RNA sequences in a heterogeneous cell populations including tissue samples and environmental samples. Riboprobes also allow to localize and assess degree of gene expression . The technique is particularly useful in neuroscience.
In situ hybridization probes
Applications of In Situ Hybridization
Generation of riboprobes
Note: [MAJR] is a Medical Subject Heading (MeSH) tag for Major Heading. The tag is used to limit the search to articles for which major subjects are represented by terms included in the NLM MeSH database.
» Brown C. In situ hybridization with riboprobes: an overview for veterinary pathologists. Vet Pathol. 1998 May;35(3):159-67. PMID: 9598579
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