Amplified Fragment Length Polymorphism (AFLP)
The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA.
How It Works
Using 3-bp selective primer extensions gives 128 possible linker combinations. Therefore, 128 subsets of genomic DNA can be readily amplified. Thus, thousands of markers can be generated quite rapidly.
Weaknesses of AFLP
» Vos P, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407-14. PMID: 7501463
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