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Study Description

This study involved evaluation of the tissue associated microbiome of the ileal pouch following surgery for ulcerative colitis (UC) or familial adenomatous polyposis (FAP). Individuals were recruited, with biopsies taken from the ileal pouch and the pre-pouch ileum, microbial DNA was extracted and sequenced using 454 pyrosequencing. Total bacterial community structure and abundance were evaluated to determine which changes were characteristic of inflammatory phenotypes including pouchitis (inflammation of the ileal pouch) and a Crohn's disease-like phenotype.

  • Study Type: Case-Control
  • Number of study subjects that have individual level data available through Authorized Access: 80

Authorized Access
Publicly Available Data (Public ftp)

Connect to the public download site. The site contains release notes and manifests. If available, the site also contains data dictionaries, variable summaries, documents, and truncated analyses.

Study Inclusion/Exclusion Criteria

Inclusion Criteria

  • Patients having undergone ileal pouch-anal anastomosis for ulcerative colitis (UC) or familial adenomatous polyposis (FAP) with ileostomy closure at least one year prior to study enrollment
  • Sufficient clinical information is available to classify patients into post-surgical outcome groups
  • Patients willing to undergo study pouchoscopy with biopsy

Exclusion Criteria

  • Pre-surgical diagnosis of Crohn's disease
  • < 18 years old

Molecular Data
TypeSourcePlatformNumber of Oligos/SNPsSNP Batch IdComment
Metagenomic Sequencing Roche 454 GS FLX Titanium N/A N/A Next generation sequencing of V1-V3 hypervariable region of 16S rRNA gene of microbial DNA
Selected publications
Diseases/Traits Related to Study (MESH terms)
Links to Other NCBI Resources
Authorized Data Access Requests
Study Attribution
  • Principal Investigator
    • Mark Silverberg, MD., PhD. Zane Cohen Centre for Digestive Diseases, Mount Sinai Hospital, Toronto, ON, CANADA
  • Funding Source
    • Genetic, serological and microbial factors related to patterns of ileal inflammation. Crohn's and Colitis Foundation of Canada, Toronto, ON, CANADA