NCBI Genome Guide Tutorial


Links of interest:
NCBI Handbook
NCBI Guides
Mouse Guide Page
Genome Glossary
NCBI Build Info
RefSeq
GSA Course
Church talk 1/13/05
Examples:
1. Identifying the list of annotated genes between two markers.
For this example we will use an obesity QTL:
Obq7: flanked by (D1Mit213, D1Mit131) peak(D1Mit76)
    A. By text search
    B. By sequence comparison (e-PCR)

2. For a given region of the mouse genome, how can I use comparative genomics to inform by gene identificationi project?
    A. What is HomoloGene
    B. Comparative Map Viewer

3. How can I determine how a clone relates to the assembly if there is no insert sequence?
    Find my Clones!

4. How do I search for ESTs expressed in certain tissues?
    EST expression

5. How do I obtain sequence flanking my gene of interest?
    Flanking sequence

6. How can I obtain ESTs for a particular gene model?
    Find my ESTs

7. What is the best way to download really huge (>100000 Kb)?
    Get my big sequence

8. How can we submit polymorphic sequences?
    Make data public

9. What is the best way to identify transcription factor binding site and regulatory sites?