Molecular Inversion Probe Assay

Introduction

Molecular Inversion Probe (MIP) Assay

is and efficient technology for large-scale Single Nucleotide Polymorphisms (SNPs) analysis. It is used for SNP discovery and genotyping. This technique produces "inverted" probes in which SNPs are introduced into tag sequences that could be analyzed using a universal sequence tag. This technology allows to perform multiplex analysis of more than 10,000 probes in a single tube can be done using standard laboratory equipment.

Molecular Inversion Probes

(originally called "padlock probes") are single-stranded DNA molecules containing two regions complementary to regions in the target DNA that flank SNP in question. Each probe also contains universal primers' sequences separated by endoribonuclease recognition site and a 20-nt tag sequence. During the assay the probes undergo a unimolecular rearrangement: they are (1) circularized by filling gaps with nucleotides corresponding to the SNP in four separate allele-specific polymerization (A, C, G, and T) and ligation reactions; (2) linearized in enzymatic reaction. As a result they become "inverted". This step is followed by PCR amplification. Further processing of the probes depends on specific assay.

How it works

Molecular Inversion Probe technology

Sample Queries

References

Circularization probes
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Last updated: 2014-09-24T13:55:49-04:00