PMC

A, 80% confluent IEC-6 monolayers were infected with Ad-Slfn3 for 48 hours (in 6 rats). B, Ad-GFP-Slfn3 infection of IEC-6 cells increased Dpp4-specific activity in lysates from these IEC-6 monolayers (in 3 rats). Error bars indicate standard errors. RLU indicates relative luminescence units.

Transcript levels of Slfn1 (A), Slfn2 (B), Slfn3 (C), Slfn5 (D), Slfn13 (E), and Slfn14 (F) in the jejunum (Jj), upper ileum (IL1), middle ileum (IL2), lower ileum (IL3), and colon (Co) of rats were determined by quantitative reverse transcriptase–polymerase chain reaction. A significant difference is indicated if the letter above one error bar is different from that of another (P < .05). Error bars indicate standard errors.

Transcript levels of villin (A), Dpp4 (B), Glut2 (C), and SI (D) were measured by quantitative reverse transcriptase–polymerase chain reaction in the mucosa of the jejunum (Jj), upper ileum (IL1), middle ileum (IL2), lower ileum (IL3), and colon (Co). Ordinate scales were matched to the ordinate scales in to facilitate interpretation. A significant difference is indicated if the letter above one error bar is different from that of another (P < .05). Error bars indicate standard errors.

Protein levels of Slfn3 (A), villin (B), Glut2 (C), SI (D), and Dpp4 (E) were measured in the jejunum (Jj), upper ileum (IL1), middle ileum (IL2), lower ileum (IL3), and colon (Co) of rats by Western blotting of mucosal lysates. Dpp4 enzyme activity (F) was measured in intestinal rat mucosal samples. A significant difference is indicated if the letter above one error bar is different from that of another (P < .05). Error bars indicate standard errors. RLU indicates relative luminescence units.