PMC

EdU incorporation in LNCaP cells exposed to normal, rest and exercise serum for 24 hours. Data is presented as mean ± SEM of 4 consecutive experiments. au (arbitrary units). π denotes a significant (p≤0.05) difference between incubation with rest and exercise serum.

A) Effect on LNCaP cells incubated for 48 hours with resting (rest) and exercise serum (exercise) from 10 individuals separately. B) Effect of the 10 individual serums on NIH3T3 cells. Data show all individuals separately (left panel) and as mean ± SEM. au (arbitrary units). π denotes a significant (p≤0.05) difference between incubation with rest and exercise serum.

A) Analysis of growth factor content in rest and exercise serum using a growth factor array approach. B) Rest/exercise serum ratio of 42 growth factors and cytokines, analyzed in multiplex. C) ELISA analysis of individual (left panel) and average (right panel) levels of serum EGF in rest and exercise serum, n = 9. D) ELISA analysis of individual (left panel) and average (right panel) levels of serum IGFBP-1 in rest and exercise serum, n = 10. Data is presented as individual levels or mean ± SEM. π denotes a significant (p≤0.05) difference between incubation with rest and exercise serum.

2×10⁶ LNCaP cells incubated for 48 hours in either rest serum or exercise serum were co-injected with NIH3T3 cells (4∶1) subcutaneously in SCID mice. A) Tumor growth curves of cells preincubated with rest and exercise serum respectively. Significant (p≤0.05) differences are denoted with π. n = 10 animals per group. B) Tumor incidence (percent of mice carrying tumors) at day 14 in the rest and exercise group. C) Scatter plot of tumor volume in rest and exercise group at day 34 after injections. D) Proliferating cells in tumors after experimental endpoint assessed by expression of proliferating cell nuclear antigen (PCNA). E) Apoptotic cells in tumors after experimental endpoint, assessed by Tunel stainings. For D) and E) data is the nr of positive cells per field, shown as mean ± SEM.

A) Effect of 24, 48 and 96 hours incubation with normal media supplemented with 10% FCS (normal), media supplemented with a pool of human serum from 10 resting individuals (rest) or serum from the same individuals after a single bout of exercise (exercise) on growth of the prostate cancer cell line LNCaP. B) Effect of incubation under the same conditions as in A) on growth of the mouse fibroblast cell line NIH3T3. C) Effect of 96 hours incubation with respective serum on the prostate cancer cell lines 22rv1 and Du145. Data is presented as mean ± SEM. au (arbitrary units). π denotes a significant (p≤0.05) difference between incubation with rest and exercise serum.