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1.
Figure 8

Figure 8. Light transmittance analysis of control and decellularized SI samples.. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

Panel A corresponds to samples decellularized using chemical agents, whereas results of the application of physical decellularization methods are shown in panel B. Non-decellularized intestine and native porcine cornea are shown as controls.

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

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2.
Figure 1

Figure 1. Histograms representing the efficiency of cell removal and tissue structure preservation as determined by DAPI (A), residual DNA quantification (B), picrosirius collagen staining (C), Gomori reticulin (D), PAS glycoprotein staining (E) and alcian blue proteoglycan staining (F).. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

Bars correspond to average values ± standard deviations.

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

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3.
Figure 7

Figure 7. Photographic images of control and decellularized SI samples using a patterned surface to estimate transparency.. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

Images correspond to control SI samples (A), and tissues decellularized with 1.5 M NaCl (B), 3 M NaCl (C), 5 M NaCl (D), 0.1% SDS (E), 0.3% SDS (F), 0.6% SDS (G), 0.1% triton X-100 (H), 0.3% triton X-100 (I), 0.6% triton X-100 (J), 10 min SC (K), 20 min SC (L), 30 min SC (M), 10 min UV (N), 20 min UV (O) and 30 min UV (P).

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

CitationFull text

4.
Figure 2

Figure 2. Scanning electron microscopy (SEM) images of control SI samples (A), and tissues decellularized with 1.5 M NaCl (B), 3 M NaCl (C), 5 M NaCl (D), 0.1% SDS (E), 0.3% SDS (F), 0.6% SDS (G), 0.1% triton X-100 (H), 0.3% triton X-100 (I), 0.6% triton X-100 (J), 10 min SC (K), 20 min SC (L), 30 min SC (M), 10 min UV (N), 20 min UV (O) and 30 min UV (P).. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

Scale bar represents 50 µm.

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

CitationFull text

5.
Figure 3

Figure 3. Picrosirius collagen fibers staining of control SI samples (A), and tissues decellularized with 1.5 M NaCl (B), 3 M NaCl (C), 5 M NaCl (D), 0.1% SDS (E), 0.3% SDS (F), 0.6% SDS (G), 0.1% triton X-100 (H), 0.3% triton X-100 (I), 0.6% triton X-100 (J), 10 min SC (K), 20 min SC (L), 30 min SC (M), 10 min UV (N), 20 min UV (O) and 30 min UV (P).. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

All scale bars represent 200 µm, with exception of the insert representing a higher magnification image (scale bar = 800 µm).

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

CitationFull text

6.
Figure 4

Figure 4. Gomori reticulin staining of control SI samples (A), and tissues decellularized with 1.5 M NaCl (B), 3 M NaCl (C), 5 M NaCl (D), 0.1% SDS (E), 0.3% SDS (F), 0.6% SDS (G), 0.1% triton X-100 (H), 0.3% triton X-100 (I), 0.6% triton X-100 (J), 10 min SC (K), 20 min SC (L), 30 min SC (M), 10 min UV (N), 20 min UV (O) and 30 min UV (P).. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

All scale bars represent 200 µm, with exception of the insert representing a higher magnification image (scale bar = 800 µm).

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

CitationFull text

7.
Figure 5

Figure 5. PAS glycoprotein staining of control SI samples (A), and tissues decellularized with 1.5 M NaCl (B), 3 M NaCl (C), 5 M NaCl (D), 0.1% SDS (E), 0.3% SDS (F), 0.6% SDS (G), 0.1% triton X-100 (H), 0.3% triton X-100 (I), 0.6% triton X-100 (J), 10 min SC (K), 20 min SC (L), 30 min SC (M), 10 min UV (N), 20 min UV (O) and 30 min UV (P).. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

All scale bars represent 200 µm, with exception of the insert representing a higher magnification image (scale bar = 800 µm).

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

CitationFull text

8.
Figure 6

Figure 6. Alcian blue proteoglycan staining of control SI samples (A), and tissues decellularized with 1.5 M NaCl (B), 3 M NaCl (C), 5 M NaCl (D), 0.1% SDS (E), 0.3% SDS (F), 0.6% SDS (G), 0.1% triton X-100 (H), 0.3% triton X-100 (I), 0.6% triton X-100 (J), 10 min SC (K), 20 min SC (L), 30 min SC (M), 10 min UV (N), 20 min UV (O) and 30 min UV (P).. From: Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering.

All scale bars represent 200 µm, with exception of the insert representing a higher magnification image (scale bar = 800 µm).

Ana Celeste Oliveira, et al. PLoS One. 2013;8(6):e66538.

CitationFull text

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