Phenotype of mice bearing Treg-specific Brg1 PM. (A–D) Survival (A), body weight at 40 days of age (B) and various organs (C, D) at ∼30 days of age. All mice were males. A and B are identical to and , respectively, except for the inclusion of Brg1F/O; FoxP3YFP-Cre point mutants (PM). Arrow in D indicates marginal infiltration in the liver. (E) Treg activation in 20- to 50-day-old mice (top) and in adult mosaic mice (bottom), which are identical to and , respectively, except for the inclusion of the PM mice. Note that in the plots at the top, the mice are not separated into different age groups as in due to limiting sample sizes of the PM mice. F/O, mosaic PM females (Brg1F/O; FoxP3YFP-Cre/+). (F) Treg abundance in adult mosaic females. The plot is identical to except for the inclusion of PM mice (Brg1F/O; FoxP3YFP-Cre/+). (G) PCR analysis of Brg1F (F) and Brg1O (O) in tail (lanes 1–2) and YFP+ Tregs (lanes 3–4) from Brg1F/O; FoxP3YFP-Cre/+ mosaic females. The two alleles were detected by a primer pair flanking the 3′-LoxP site in Brg1F and that amplifying a fragment specifically present in Brg1O, respectively. The asterisk indicates co-amplified internal controls. The data indicate efficient Brg1F deletion concomitant with Brg1O conversion. The assays were performed essentially as described ().